New additions Conor Wilkinson and Alex Lacey will be part of the Gillingham squad that travels to the Keepmoat Stadium despite carrying injuries.
Striker Wilkinson, who arrived from Bolton on a two-year deal, is suffering with an ankle issue while Lacey (knee) will face a late fitness test.
Aaron Morris remains a long-term absentee for the Gills with a knee problem.
Meanwhile, midfielder Lee Martin will be the new captain for the visitors after being handed the armband for the forthcoming campaign.
Ady Pennock is expected to hand full league debuts to a number of signings over the summer with his new look defence hoping to be more water tight than last season.
Tomas Holy is expected to be given the goalkeeper’s jersey ahead of Stuart Nelson despite a shaky pre-season campaign as The Gills look to get their League One season underway with a decent result.
Nelson may also miss out on being in the eighteen man squad with Tom Hadler set to deputise for Holy.
With an emphasis on being a more physical side this season, Pennock will expect more from his Gills side that underperformed in 2016/17.
Doncaster will be without summer signing Alex Kiwomya for the visit of Gillingham.
Former Chelsea striker Kiwomya, who signed a three-year deal with newly-promoted Rovers in June, is expected to be out for two months due to a back-related calf injury.
Ross Etheridge will be out for another three months as he recovers from a broken ankle sustained during training in early July, but fellow goalkeeper Ian Lawlor is expected to shake off a knock.
Latest signings Issam Ben Khemis and Rodney Kongolo are both in contention, as are fellow summer recruits Niall Mason, Ben Whiteman and Danny Andrew.